Work Package 4

LARGE-SCALE PHENOTYPING OF CELLULAR AND ANIMAL MODELS OF RSDs AND CSDs

Work package leader:

Anna Teti (UNIVAQ)

Objectives:

• To evaluate, validate and standardize core technologies that will be used for deep phenotyping of the models.
• To provide qualitative & quantitative data on changes in cellular phenotype, morphology and differentiation.
• To deep phenotype the bone and/or cartilage defects in both cellular and animal models of RSDs and CSDs and interpret phenotypic variability.

Partners involved in WP4:

• University of Newcastle
• University of L’Aquila
• University of Manchester
• University of Pavia
• Consiglio Nazionale delle Ricerche
• Institut National de la Santé et de la Recherche Médicale
• Certus Technology Associates Limited
• Charité – Universitaetsmedizin Berlin
• University Medical Center Hamburg Eppendorf
• Evercyte
• University Hospital of Cologne
• University of Freiburg
• University of Antwerp

Deliverables 

• D4.1 Agreed protocols for phenotyping and radiographic imaging of animal models
• D4.2 Agreed protocols for histochemistry, IHC and histomorphometry of cartilage and bone
• D4.3 Agreed protocols for culture conditions of in vitro and ex vivo models used by different partners and method of analysis
• D4.9 Collection of gold-standard representative images and key scientific and phenotypic findings