Wax embedding of cartilage tissue

Sample preparation

Fix tissues in ice cold 4% PFA or 95%EtOH/5%HAc for 48h overnight. Decalcify in 20% EDTA pH7.4 for 1 (up to 2 weeks of age) to 2 weeks (3-9 weeks of age). Rinse the EDTA off under running tap water for 1h before embedding.

Processor program

70% EtOH 1h

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90% EtOH 45min

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95% EtOH 45min

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100% EtOH 45min

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100% EtOH 45min

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100% EtOH 45min

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xylene 30min

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xylene 30min

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xylene 30min

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paraffin wax 1h

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paraffin wax 1h

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embed in the same wax type using metal embedding molds